Fabrication of impedimetric sensors for label-free Point-of-Care immunoassay cardiac marker systems, with microfluidic blood flow delivery, and results telemetry to PDA

Recent work has demonstrated nanopatterning of gold, using a Focussed Ion Beam, and similarly, nanotemplating gold, for direct protein attachment, useful for alternative sensing techniques. The impedance changes due to myoglobin capture by antimyoglobin immobilised on gold, have been measured, in a 3 electrode electrochemical cell. The impedance changes reach an end-point level after 15-20 minutes. Two electrode planar sensors have been fabricated in gold/titanium on PI. Microfluidic delivery of blood/serum sample to the sensor was investigated, with HF-etched test structures in glass. Sensor microfluidic structures will be replicated in PMMA. Point-of-Care cardiac marker systems are under development, incorporating the novel elements of RF data transmission to PDA, and label-free impedimetric immunoassay sensing.